DNA

Part:BBa_K5017011:Design

Designed by: Christos Kalogeropoulos   Group: iGEM23_Patras-Med   (2023-10-11)


Optimized_4_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 382
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part taking into account the energy of binding scFv to mesothelin antigen.


Source

This part was designed by the software DiffAb, a diffusion-based generative model for antigen-specific antibody design and optimization.

References